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KMID : 0921620160460020063
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Journal of Bacteriology and Virology
2016 Volume.46 No. 2 p.63 ~ p.70
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Development and Evaluation of Indirect ELISA for Detection of Antibodies to Getah Virus in Horse Serum
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Lee Seung-Heon
Yang Dong-Kun
Kim Ha-Hyun
Jo Hyun-Ye
Choi Sung-Suk
Cho In-Soo
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Abstract
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Getah virus (GETV) is a member of the genus Alphavirus in the family Togaviridae. GETV infection can occur in a wide range of vertebrate species, and the virus has been known for a pathogen of horses and pigs. To rapidly and accurately diagnose GETV infection of a racehorse, an indirect ELISA (I-ELISA) was developed in the present study for detection of antibodies to GETV in serum samples. To evaluate the developed I-ELISA, a total of 240 serum samples from Thoroughbred racehorses raised in Korea were screened in parallel by a serum neutralization (SN) test. The developed I-ELISA exhibited an efficacy comparable to that of the SN test in terms of a high diagnostic sensitivity (86.3%) and specificity (94.5%) at a cut-off absorbance value of 0.25. In addition, our results showed that the developed I-ELISA had a significant correlation with the SN test (r = 0.91; p < 0.05). Taken together, our findings suggest that the I-ELISA developed in this study is a valuable diagnostic tool for the screening of horses suspected to be infected with GETV.
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KEYWORD
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Getah virus, ELISA, Racehorse
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